Orbitrap Script 2.0
Analysis of MS phosphorylation
- Script accepts psms text data files in tab separated format
- Script accepts single zip file created according to following rules:
- Zip file includes directories with sample names
- Every directory has single file for qualitative analysis and number of files for quantitative analysis
- Name of files for qualitative analysis and for quantitative analysis have to be distinguishable by unique identifiers, which can be specified below
- At least 2 distinct files have to be supplied
- For overview of accepted format see example files from sample_files.zip from human Dishevelled 2
- All parameters have to be specified
- Script produces 3 files.
- Report for qualitative analysis with sample characteristics and peptide data
- Summary of phosphorylations from every sample
- Quantitative analysis of individual cluster phosphorylations
- Result files are kept on server for 24 hours